In Chemico Skin Sensitisation: Direct Peptide Reactivity Assay (DPRA)

(OECD Test Guideline 442C)

     The DPRA test is accepted at a regulatory level and compliant with the CLP Regulation 1272/2008, and REACH (Registration, Evaluation, Authorisation and restriction of Chemicals). This animal-free, in vitro test is also compliant with the EU Cosmetics Regulation 1223/2009.   

     The DPRA test classifies a test formulation or ingredient in one of four classes of reactivity (No or Minimal, Low, Moderate or High). Test formulation or ingredients that are of Low, Moderate or High reactivity class are identified as skin sensitisers* (Category 1 Hazards) according to the UN Globally Harmonised System (GHS). 

  • Overview

Turnaround Time

4 - 6 weeks


Our test prices are dependent on the quantity of test items.

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*A skin sensitiser is a substance that will lead to an allergic response following skin contact.

     The adverse outcome pathway leading to skin sensitisation involves a number of key events. DPRA test method addresses the molecular initiating event – reactivity with proteins – by quantifying the reactivity of test items towards model synthetic peptides containing either cysteine or lysine.   


In Vitro and In Chemico methods to predict skin sensitisation. The Adverse Outcome Pathway for skin sensitisation initiated by covalent binding to proteins.